气相色谱-四极杆飞行时间串联质谱的使用与维护保养

Use and Maintenance of Gas Chromatography-quadrupole Time-of-flight Mass Spectrometry

中文关键词:  气相色谱-四极杆飞行时间串联质谱  使用  维护保养

英文关键词:GC-QTOF  use  maintenance

中文摘要:

      高分辨气相色谱-四极杆飞行时间串联质谱（GC-QTOF）可以在单飞行时间质谱（TOF）采集模式下，利用采集到的精确质量数离子对未知物进行分析和结构鉴定.GC-QTOF主要利用低分辨的美国国家标准与技术研究院质谱库（NIST）和高分辨的个人自建库（PCDL）进行搜索匹配，也能利用QTOF的碎片离子来倒推未知化合物，其主要利用分子结构推导软件（MSC软件）和Chemspiders（web）等数据库来搜索匹配.在做好仪器操作使用的同时也要经常性对仪器进行维护保养，确保仪器处于良好的使用状态，保证检测数据的准确、有效.主要介绍了安捷伦GC-QTOF的使用与维护保养.

英文摘要:

      High resolution gas chromatography-quadrupole time-of-flight mass spectrometry(GC-QTOF) can be acquired in single mode time-of-flight mass spectrometry(TOF), with the use of accurate mass number of collected ions to analyze unknown compound, and carry out structure identification, which are mainly based on the use of the National Institute of Standards and Technology(NIST) library of the low resolution and personal compound database and library (PCDL) of high resolution. GC-QTOF also can analyze unknown compounds by QTOF fragment ions, which are mainly based on the use of the molecular structure of software (MSC) and Chemspiders database (web) and so on. Besides to operate the instrument correctly with regular maintains,and make the instrument in good working condition, in order to get the accuracy and effectiveness of the test data. The use and maintenance of GC-QTOF from Agilent is introduced.