高效液相色谱法测定林可霉素利多卡因凝胶中盐酸利多卡因含量

Determination of Lidocaine Hydrochloride in Lincomycin Hydrochloride and Lidocaine Hydrochloride Gel by High Performance Liquid Chromatography

中文关键词:  高效液相色谱法  利多卡因  林可霉素利多卡因凝胶

英文关键词:HPLC  lidocaine  lincomycin hydrochloride  lidocaine hydrochloride gel

中文摘要:

      建立了高效液相色谱法（HPLC）测定林可霉素利多卡因凝胶中盐酸利多卡因含量的方法.优化后的试验条件如下：色谱柱为Agilent 5 Tc-C18（2）（250 mm×4.6 mm，5 μm），流动相为0.05 mol/L硼砂溶液（pH=6.0）-甲醇-乙腈（体积比为68：19：13），紫外检测波长为214 nm，流速1.0 mL/min，柱温30℃，进样量20 μL.试验结果表明：利多卡因的线性范围为40.1~240.6 μg/mL（r =0.999 4），加样回收率为97.6%.建立的方法操作简便，可用于制剂中盐酸利多卡因的质量控制.

英文摘要:

      A method for the determination of lidocaine hydrochloride in lincomycin hydrochloride and lidocaine hydrochloride gel by high performance liquid chromatography(HPLC)is set up. Agilent 5 Tc-C18(2)(250 mm×4.6 mm, 5 μm)column was used. The mobile phase consisted of 0.05 mol/L Borax (adjusted to pH 6.0 with phosphoric acid)-methanol-acetonitrile(68:19:13). The UV detection wavelength was at 214 nm, with a flow rate of 1 mL/min and the column temperature was 30℃. The linearity was good for lidocaine within the range of 40.1~240.6 μg/mL(r=0.999 4), the sample recovery rate was 97.6%. The established method is operative and simple, and can be used for the quality control of lidocaine hydrochloride in preparations.