HPLC法测定不同施肥模式栽培的金线莲中黄酮类成分

Determination of Flavonoids in Anoectochilus Roxburghii (wall) Lindl Cultivated with Different Modes by HPLC

中文关键词:  金线莲  高效液相色谱  超声-微波协同提取  黄酮  氮、磷、钾复合肥  硫、磷复合肥

英文关键词:A.roxburghii  HPLC  ultrasonic-microwave synergistic extraction  flavonoid  NPK  SP

中文摘要:

      HPLC法测定不同施肥模式栽培的金线莲中3种黄酮类成分：槲皮素、山奈酚和异鼠李素.超声-微波协同提取法提取4种不同施肥模式下金线莲中的黄酮类成分.HPLC-电喷雾离子化法/质谱（HPLC-ESI-MS）对黄酮类化合物进行定性分析.HPLC分析得槲皮素在0.25~20.0 μg/mL（r=0.999 2）、山奈酚在0.25~20.0 μg/mL（r=0.999 5）、异鼠李素在1.0~80.0 μg/mL（r=0.999 0）范围内峰面积与浓度呈良好的线性关系，不同栽培方法的金线莲药材中槲皮素、山奈酚、异鼠李素的质量分数分别在151.8~240.0、47.2~88.2、16.8~29.8 μg/g之间.方法简单、精确，快速，可为科学评价不同培养模式金线莲质量提供依据.栽培过程施加氮、磷、钾（NPK）复合肥及添加腐植素和微量元素，能提高金线莲中3种黄酮类成分的含量.栽培过程施加硫、磷（SP）复合肥，槲皮素、山奈酚和异鼠李素的质量分数分别下降了2.3%、17.2%和18.8%.结果表明采用不同施肥模式直接影响到药材中的药效物质的含量.

英文摘要:

      Three flavonoid compounds(quercetin, kaempferol and isorhamnetin)in Anoectochilus roxburghii cultivated with four different modes were determined by HPLC. The above metioned compounds were extracted by ultrasonic-microwave synergistic extraction method and the quantitative and qualitative analysis were separately analyzed by HPLC and HPLC-ESI-MS. Quercetin, kaempferol and isorhamnetin had good linear relationship in the range of 0.25~20.0(r=0.999 2), 0.25~20.0(r=0.999 5), and 1.0~80.0(r=0.999 0), respectively. The contents were within 151.8~240.0、47.2~88.2、16.8~29.8 μg/g, respectively. The method is a simple, accurate and fast method for the quality evaluation of A. roxburghii cultivated with different modes. The contents of three flavonoid compounds increased effectively in A. roxburghii with the addition of nitrogen, phosphorus, potassium(NPK) compound fertilizer in cultivation and decreased 2.3%, 17.2% and 18.8% respectively in A. roxburghii with the addition sulfur-phosphorus(SP) compound fertilizer, this finding provides scientific guidance for planting A. roxburghii.