树莓中花色苷的超声提取工艺以及抗氧化活性研究

Ultrasonic-Assisted Extraction of Anthocyanins from RubusCorchorifolius L. f and Antioxidant Activity

中文关键词:  树莓  花色苷  响应面法  抗氧化

英文关键词:RubusCorchorifolius L. f  anthocyanins  response surface method  antioxidant

中文摘要:

      在单因素试验的基础上选择提取次数、提取试剂、料液比、超声时间4个因素为自变量，以树莓花色苷的提取率为响应值，进行Box-Behnken中心组合试验设计，采用响应面法（RSM）评估了这些因素对花色苷提取率的影响.并分别采用1，1-二苯基苦基苯肼（DPPH）和2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）法对花色苷的抗氧化活性进行研究.结果表明，超声辅助提取树莓中花色苷的最佳工艺条件为：超声提取3次，料液比1∶46 g/mL，超声时间44 min，在此条件下预测花色苷的得率为6.79%.花色苷对于DPPH的清除率达到13.68 μmol/g，对于ABTS的清除率达到8.37 μmol/g.

英文摘要:

      Based on single factor experiments, extraction times, extraction reagent, solid-liquid ratio, ultrasonic time were selected for the Box-Behnken central composite design. Response surface method was employed to study the effect of these factors on the yield of anthocyanins and the antioxidant activity of anthocyanins. 2,2-diphenyl-1-picrylhydrazyl(DPPH) and 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)method were used, respectively to study the antioxidant activity of anthocyanins in raspberry. The results indicated that optimal conditions were:ultrasonic extraction for 3 times, 1:46 g/mL as the ratio of liquid to solid with 44 min extraction. Under these optimized conditions, the extraction yield of anthocyanins was up to 6.79%. The clearance of DPPH was 13.68 μmol/g and the clearance of ABTS was 8.37 μmol/g. The study provides scientific and theoretical basis for the research and utilization of RubusCorchorifolius L. f.