聚多巴胺仿生纳米微球用于构建电化学免疫传感器

Fabrication of Electrochemical Immunosensor Based on Biomimetic Polydopamine Nano-microspheres

中文关键词:  仿生聚合  石墨烯  多巴胺  电催化

英文关键词:biomimetic polymerization  graphene  dopamine  electro-catalysis

中文摘要:

      利用多巴胺仿生聚合方法制备了具有良好生物相容性的聚多巴胺纳米微球,并在其表面原位合成银纳米颗粒. 复合物微球具有良好的催化还原H₂O₂的性能以及良好的结合生物分子的能力. 将制备的复合物微球作为标记物,将氨基化石墨烯作为基底材料,构建了检测人免疫球蛋白(IgG)的夹心型电化学免疫传感器. 运用循环伏安法和计时电流法对构建的电化学免疫传感器进行了性能分析,并对实验条件进行了考察优化. 在最佳的实验条件下,免疫传感器的线性范围是0.1 pg/mL~15 ng/mL,检出限为0.025 pg/mL.

英文摘要:

      In this work, polydopamine nano-microspheres with good biocompatibility were prepared by the biomimetic dopamine self-polymerization. Silver nanoparticles were modified on the polydopamine microspheres. The composite microspheres show good catalytic performance towards the reduction of H₂O₂ and binding capacity with biomolecules. An electrochemical immunosensor was proposed using amino functional graphene as the substrate material and composite microspheres as labels. Cyclic voltammetry and chronoamperometry were used to investigate the performance of electrochemical immunosensor. Under optimal conditions, the linear range of the immunosensor was 0.1 pg/mL~15 ng/mL with a detection limit of 0.025 pg/mL.