超高效液相色谱-串联质谱法同时测定牛肉和牛肝中5种β2-受体激动剂

Determination of β2-Agonist in Beef and Ox Liver with UPLC-MS/MS

中文关键词:  β2-受体激动剂  UPLC-MS/MS  牛肉  牛肝  残留测定

英文关键词:β2-agonist  UPLC-MS/MS  beef  ox liver  residue determination

中文摘要:

      建立了牛肉和牛肝中克仑特罗(clenbuterol)、莱克多巴胺(ractompamine)、沙丁胺醇(salbutamol)、西马特罗(cimaterol)和特布他林(terbutaline)的超高效液相色谱-串联质谱同时快速检测方法。样品经酶解，固相萃取技术（SPE）净化，多反应监测模式（MRM）下进行定性和定量分析。标准工作溶液在0.2~10μg/kg浓度范围内，5种受体激动剂线性关系良好，相关系数R^{2均大于0.99。方法的检出限为0.2μg/kg，定量限为0.5μg/kg。在0.5、1、2μg/kg的添加浓度下，回收率在78.2%~114.2%之间，相对标准偏差在2.0~16.0%之间。方法具有快速、经济、准确等特点，适合于牛肉和牛肝中β2-受体激动剂的快速定量、定性检测。}

英文摘要:

      A method to detect the residues of clenbuterol, ractompamine, salbutamol,cimaterol and terbutaline in beef and ox liver is developed. The sample is extracted after enzyme digestion, and purified and enriched using solid phose extraction，then analyzed qualitatively and quantitatively under the MRM mode. The method has a lower limit of detection of 0.2μg/kg, and a quantitation of 0.4μg/kg. The average recoveriy of standard addition levels (0.5,1,2μg/kg) ranged in 78.2％~114.2% with relative standard deviations between 2.0％~16.0％.The method is fast, economic, accurate and sensitive,it is suitable for the rapid qualitative and quantitative detection of β2-agonist in beef and ox liver.