液相色谱-电喷雾电离/离子阱质谱法分析金线莲中3-吡啶甲醇

Analysis of 3-pyridinemethanol in Anoectochilus Roxburghii by Liquid Chromatography-Electrospray Ion Trap Mass Spectrometry

中文关键词:  金线莲  固相萃取  液-质联用  3-吡啶甲醇

英文关键词:anoectochilus roxburghii  SPE  LC-MS  3-pyridinemethanol

中文摘要:

      应用现代提取技术超声-微波协同萃取金线莲中的3-吡啶甲醇，采用固相萃取技术对样品进行前处理，高效液相色谱法-电喷雾电离/离子阱质谱法(HPLC-ESI/MS)对提取物中3-吡啶甲醇进行测定和鉴别.色谱条件：Agilent TC-C₁₈色谱柱(5 μm，4.6×250 mm)，流动相：甲醇-0.02 mol/L乙酸铵(5∶95,V/V)，流速：1 mL/min，检测波长：260 nm.结果表明峰面积与3-吡啶甲醇在浓度1~10 μg/mL范围内呈良好线性关系.回收率在92.0%~96.2%之间，相对标准偏差为2.36%.该法简便、准确、快速.

英文摘要:

      3-pyridinemethanolwas extracted from Anoectochilus roxburghiiby the ultrasonic-microwave assisted extraction(UMAE). After pretreatment using solid phase extraction(SPE) technology, 3-pyridinemethanolwas identified and determined by high performance liquid chromatography-electrospray ion traps mass spectrometry. The chromatographic separation was achieved on an Agilent TC-C₁₈column（5 μm，4.6×250 mm）using methanol -0.02 mol/L ammonium acetate（5∶95,V/V）as the mobile phase at a flow rate of 1.0 mL/min, and 260 nm as the detection wavelength. The analyte showed good linear regression relationship between peak area and concentrations in the range of 1~10 μg/mL (r=0.9996).The recovery rate was 92.0%~96.2% with an RSD of 2.36%. The proposed method is simple, accurate and rapid.