铬天青S分光光度法测定甲烷氧化菌素的研究

Study on Determination of Methanobactin by Spectrophotometry Using Chrome Azure S

中文关键词:  铬天青S  甲烷氧化菌素  乙二胺四乙酸二钠  十六烷基三甲基溴化铵

英文关键词:chrome azure S(CAS )  methanobactin  EDTA  hexadecyl trimethyl ammonium bromide ( HDTMA )

中文摘要:

      铬天青S（CAS）在十六烷基三甲基溴化铵存在条件下能与Cu产生蓝紫色的络合物，乙二胺四乙酸二钠（EDTA）和甲烷氧化菌素（mb）可以夺取络合物CAS-Cu中的Cu而产生颜色变化.采用分光光度法绘制二者的铜络合曲线，得出回归方程，进而计算出mb的EDTA相当量，最终设计一种检测甲烷氧化菌素的测定方法.其最大吸收波长为605 nm，EDTA线性回归方程为Y=-3.5X+0.00536.样品分析结果说明方法灵敏度较好,具有准确、重现性好的优点.

英文摘要:

      Chrome azure S (CAS) and Cu ions form blue-purple complex under the existence of hexadecyl trimethyl ammonium bromide. EDTA and methanobactin(mb) may change the color of the CAS-Cu complex, respectively, because of the copper-binding ability. In the spectrophotometric method, the copper-binding curve of EDTA and methanobactin can be drawn, and the relative concentration of mb to EDTA can be calculated by a regression equation. Now a spectrophotometric method to detect methanobactin is designed, the maximum absorbance is 605 nm,and linear regression equation is Y=-3.5X+0.00536，and R²=0.9946, showing the linear relationship is good. The test results of practical samples show that this method is simple, sensitive, accurate and reproducible.