固相萃取-气相色谱法测定茶叶中多种有机磷农药

Determination of Organophosphorus Pesticide Residues in Tea by Solid-phase Extraction Gas Chromatography

中文关键词:  固相萃取  气相色谱  茶叶  有机磷  农药残留

英文关键词:organophosphorus  pesticide residue  SPE  gas chromatography  tea

中文摘要:

      建立了固相萃取-气相色谱法同时测定茶叶中16种有机磷农药残留的方法.样品用丙酮超声提取后，经自制的N-丙基乙二胺(PSA)/活性炭固相萃取柱净化，以混合溶剂乙腈/甲苯(V(乙腈)∶V(甲苯)＝1∶3)洗脱，采用气相色谱-氮磷检测器(GC-NPD)测定.方法的回收率在62.8％~109.1％之间，相对标准偏差小于14.7％，16种有机磷农药的检出限在0.01~0.16 mg/kg之间.方法快速简便、经济实用.

英文摘要:

      A method for the determination of 16 organophosphorus pesticide residues in tea by Gas Chromatography - Nitrogen Phosphorus Detector(GC-NPD) with ultrasonic extraction was established. Tea samples were extracted with 10 mL of acetone, and then cleaned by homemade PSA/activated carbon SPE cartridge and eluted with acetonitrile-toluene (1∶3,V/V). 16 organophosphorus pesticides were well separated and detected with high repeatability and precision. The recovery rate of the method was between 62.8％ and 109.1％. The relative standard deviation was less than 14.7％. The limit of detection of 16 organophosphorus was between 0.01 mg/kg and 0.16 mg/kg. The method is reliable rapid and is, suitable for the analysis of pesticide residues in real samples.