营养保健鸡蛋中α-亚麻酸含量测定

Determination of Content of α-Linolenic Acid in Nutritional Eggs

中文关键词:  营养保健鸡蛋  α-亚麻酸  毛细管气相色谱

英文关键词:nutritional eggs  α-linolenic acid  capillary gas chromatography

中文摘要:

      采用毛细管气相色谱法测定了营养保健鸡蛋中α-亚麻酸的含量.营养鸡蛋以盐酸进行酸水解提取脂肪，脂肪样品以0.5 mol/L的氢氧化钾-甲醇溶液进行甲酯化反应，以FFAP毛细管气相色谱柱程序升温，氢火焰离子化检测器测定鸡蛋样品衍生化产物中α-亚麻酸甲酯的含量.方法测定α-亚麻酸甲酯含量的日内和日间精密度分别为1.56%和2.72%，加标回收率为97.3%（相对标准偏差为2.9%），待测营养保健鸡蛋中α-亚麻酸的含量为0.49%.方法精密度和准确度良好，操作较简便，适用于营养保健鸡蛋样品中α-亚麻酸的测定.

英文摘要:

      Content of α-linolenic acid in nutritional eggs has been determined using capillary gas chromatography. The total fat in nutritional eggs was extract by hydrochloric acid for acid hydrolysis. Then the extract of the total fat was treated with a 0.5 mol/L potassium hydroxide―methanol solution for methyl esterification. The esterified sample, including α-methyl linolenate, was finally determined by a capillary gas chromatograph―flame ionization detector (FID), using a FFAP capillary column with temperature programming. The intro-day and inter-day precision of the method were 1.56% and 2.72%, respectively. The average recovery rate of the standard amount added to the sample was 97.3% (RSD was 2.9%). The content of α-linolenic acid in the detected nutritional egg was 0.49%. The result showed that the developed method was accurate, repeatable and easy to operate. It is suitable for the determination of α-linolenic acid and other polyunsaturated fatty acid in nutritional eggs.