一种液相色谱分离与质谱鉴定白血病K562细胞蛋白的方法

A Liquid Separation and MALDI-TOF MS Detection Method for Leukemia K562 Cell

中文关键词:  高效液相色谱,基质辅助电离解析时间飞行质谱,K562细胞,蛋白质组

英文关键词:high performance liquid chromatography(HPLC),matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS),K562 cell line, proteome

中文摘要:

      分别通过3种色谱模式：反相高效液相色谱（RPLC）、弱阴离子交换-反相高效液相色谱（WAX-RPLC）和排阻-反相高效液相色谱（SEC-RPLC）对K562细胞的蛋白质进行分离，收集的色谱馏分采用基质辅助电离解析时间飞行质谱（MALDI-TOF MS）进行鉴定后，比较所获得的蛋白质数据.结果显示：当选择SEC-RPLC模式构建K562细胞蛋白质图谱时，检测到的蛋白质数目最多，信息最为全面.此法的建立为白血病的临床研究提供了一种有效的手段.

英文摘要:

      The proteins of K562 cell line were separated by three modes of chromatography, the chromatographic fractions were collected and then detected by MALDI-TOF MS. From comparing the date of those separation modes a conclusion can be made: Using SEC-RPLC separation mode to establish the protein finger print of K562 cell line, more than 120 kinds of proteins from 2 KD to 130 KD can be detected. The detected proteins are the most and the required information is also most comprehensive by using this separation mode. The establishing of this method provides a valid way for clinical study of leukemia.