AuI2-分光光度法测定北豆根总碱含量

Spectrophotometric Determination of Total Alkaloids in Beidougen Capsule with AuI2-

中文关键词:  AuI₂^-  北豆根碱(BA)  缔合微粒  分光光度法

英文关键词:AuI₂^-  beidougen alkaloid(BA)  association particle  spectrophotometry

中文摘要:

      研究发现5.5×10^-2 mol/L HCl-2.8×10^-3 mol/L KI-2.4×10^-4 mol/L HAuCl₄体系的最大吸收峰在370 nm,当有北豆根碱(BA)存在时,由于形成(BA-AuI₂)_n缔合微粒,在538 nm处有一吸收峰,BA浓度在0.30~18 mg/L范围遵循Lamber Beer定律,其摩尔吸光系数ε_{538 nm}为3.57×10⁴ L/(mol·cm)(γ=0.9992,n=6).据此建立了一个测定北豆根制剂中的北豆根总碱含量的分光光度法.该法灵敏、简便、快速、准确、重复性好,平均回收率为98.0%,相对标准偏差RSD=2.06%(n=5).

英文摘要:

      In the medium of 5.5×10^-2 mol/L HCl, there is a max absorption peak at 370 nm for the system of 2.8×10^-3 mol/L KI-2.4×10^-4 mol/L HAuCl₄. A spectrophotometric method was proposed for the determination of alkaloids in Beidougen(BA) capsule, based on the formation of BA-AuI₂^- association particles which have a absorption peak at 538nm. The linear range is 0.3~18 mg/L(γ=0.9992,n=6), with a mole absorptivity of 3.57×10⁴ L/(mol/cm). This method has convenience, rapidity, accuracy and good recovery. It was applied to the determination of total alkaloids in Beidougen Capsule with satisfactory results.