烟碱-磷钼杂多酸缔合微粒体系的光谱研究及分析应用

Spectral Properties of Nicotine-PMo Heteropoly Acid and Its Analytical Application

中文关键词:  烟碱  磷钼杂多酸  缔合微粒  分光光度法

英文关键词:Nicotine  phosphomolybdenum heteropoly acid  association particle  spectrophotometry

中文摘要:

      采用分光光度法和共振散射光谱法研究了小烟碱 磷钼杂多酸缔合微粒体系.在波长300～800nm范围内,波长愈短缔合微粒体系的吸光度值愈大,在400nm处的吸光度与烟碱浓度在0.50～50.0μg/mL范围成良好线性关系,检出限(3σ)为0.1μg/mL.据此建立了一个测定烟草中烟碱含量的分光光度法.该法灵敏、简便、快速、准确、重复性好,平均回收率为99%～103%,相对标准偏差RSD为0.9%～1.3%.共振散射光谱研究结果表明,该体系存在烟碱-磷钼杂多酸缔合微粒,该微粒在420nm处存在共振散射效应.

英文摘要:

      The association particle systems of nicotine (NT)-PMo heteropoly acid were studied spectrophotometrically and resonance scattering spectroscopitrically. The absorbance values decreases with the wavelength in the range of 300-800 nm. The A_{400 nm} is linear to the nicotine concentration in the range of 0.16～24 μg/mL. Its detection limit is 0.08 μg/mL NT. A new spectrophotometric method has been proposed for the determination of NT in real samples, with some advantages including high sensitivity, simplicity, rapidity and accuracy. The means recovery is 100.5%. The RSD is 1.00%(n=5). The results of resonance scattering spectra show that there are (NT-PMo)n association particles which exhibit resonance scattering effect at 420 nm.