HPLC法测定黄芪中黄酮类成分和黄芪甲苷的含量

Determination of Astragaloside Ⅳ and Flavoniod from Radix Astragali by HPLC

中文关键词:  黄芪  黄芪甲苷  黄酮类  高效液相色谱

英文关键词:radix astragali  astragaloside Ⅳ  flavonoid  HPLC

中文摘要:

      比较黄芪对照药材和甘肃省近20个乡镇大面积种植的黄芪中黄芪甲苷和2个黄酮类化合物的含量,确定甘肃黄芪质量评价指标 采用Kromasil ODS-1色谱柱,甲醇-水为流动相,梯度洗脱,流速为1.0mL/min,检测波长254nm测定黄酮类成分的含量;乙腈-水为流动相,等度洗脱,流速为1.0mL/min,检测波长为200nm测定黄芪甲苷的含量.黄酮类成分毛蕊异黄酮的线性范围为2.350×10^-3～4.700×10^-2μg,平均回收率为98.34%,RSD为2.00%;芒柄花素线性范围为:2.325×10^-3～4.650×10^-2μg,平均回收率为96.53%,RSD为1.63%;黄芪甲苷线性范围为1.215～6.075μg,平均回收率为101.47%,RSD为2.21%.该方法简便,可靠,准确,具有一定的实用性,可用于黄芪药材质量控制.

英文摘要:

      To establish the evaluation of quality standard of Radix Astragali according to their contents of Astragaloside Ⅳ and two flavoniods in Radix Astragsli from Gansu province and the standard of Radix Astragali. High-performance-liquid-chromatograph(HPLC) on a Kromasil ODS column (5 μm,4.6 mm×250 mm) with water-methanol gradient elution, at column temperature of 25℃, flowrate of 1.0 mL/min and wavelength at 254 nm for determenation of two flavoniods; with acetiontrile-water isocratic elution, at column temperature of 25℃, flowrate of 1.0 mL/min and wavelength at 200 nm for determenation of Astragaloside Ⅳ. Linear ranges of Astragaloside Ⅳ, 7-hydroxy-4'-methoxyisoflavone and 7,3'-dihydroxy-4'-methoxy-isoflavone were 1.215～6.075 μg, 2.325×10^-3～4.650×10^-2 μg and 2.350×10^-3～4.700×10^-2 μg respectively. The average recovery were 101.47%, 96.53% and 98.34% respectively, and relative standard deviations were 2.21%, 1.63% and 2.00% respectively. This method was practical and provided a new idea to study the quality standard of traditional Chinese drug.