聚天冬氨酸硅质固定相的研制及应用

Synthesis and Application of Poly(D, L-aspartic Acid)-Silica Stationary Phase

中文关键词:  HPLC  D,L-天冬氨酸  固定相  蛋白质

英文关键词:HPLC  D, L-aspartic acid  stationary phase  protein

中文摘要:

      D,L-天冬氨酸在浓磷酸的存在下加热聚合生成聚琥珀酰亚胺,此产物能与氨丙基硅胶快速反应生成聚琥珀酰亚胺硅胶,然后再水解生成聚天冬氨酸硅质固定相.并对反应条件进行了优化.实验表明,聚天冬氨酸固定相对蛋白质有较好的分离能力和选择性.

英文摘要:

      In the presence of concentrate H₃PO₄ the poly(succinimide) was quantitatively polymerized by heating D,L-aspartic acid. The product quickly reacted with aminopropyl-silica, and then was hydrolyzed to poly(aspartic acid)-silica. It was found that the poly(aspartic acid)-silica stationary phase had good ability to separate proteins. The reaction conditions were also optimized during the investigation.