微加工芯片式流通池在顺序注射可更新表面反射光谱检测中的应用

Studies on a Sequential Injection Renewable Surface Reflectance Spectrophotometric System Using a Microfabricated Microchip Flow Cell and Its Application

中文关键词:  微加工芯片式流通池  顺序注射可更新表面  反射光谱法  锌

英文关键词:microfabricated microchip flow cell  sequential injection renewable surface technique  reflectance spectrophotometry zinc

中文摘要:

      提出了一种以湿法蚀刻技术制备的用于流动注射(FI)或顺序注射(SI)进样可更新表面检测的芯片式流通池,并将此流通池与SI系统及检测器相匹配,用于顺序注射可更新表面(SIRST)反射光谱法检测.流通池由两片玻璃封接而成,流通池通道蚀刻在玻璃基片上.流通池通过多股双岔光纤分别与光源、检测器相耦合,以实现对微珠表面的反射光谱法检测.将此SIRST反射光谱法检测系统用于对锌的检测,并测定人发试样中微量锌的含量.

英文摘要:

      A sequential injection renewable surface reflectance spectrophotometric system employing a microchip flow cell was developed. The double layer microchip flow cell with a weir structure for bead collection was fabricated on glass chips using a standard photolithographic procedure followed by thermal bonding. The flow cell was coupled to a sequential injection system and an optical fiber photometric detection system. The SI-RST analysis system was applied to the determination of trace amounts of zinc by reflectance spectrophotometry. The analyte reacted with zinc sorbed on Polysorb C18 beads trapped in the flow cell. The proposed method was successfully applied to the determination of zinc in human hair.