高效液相色谱法测定血清中茶碱浓度

Determination of Theophylline in Human Serum by High-Performance Liquid Chromatography

中文关键词:  茶碱  高效液相色谱  血清

英文关键词:theophylline  high-performance liquid chromatography  serum

中文摘要:

      采用高效液相色谱,分析柱:3μm,3.3cm×4.6mm,I.D(PerkinElmer,USA);预柱:10μm,1cm×2.1mm,I.D(PerkinElmer,USA);以乙酰氨基酚为内标对氯仿异丙醇(95:5,V/V)提取样品进行了分析.流动相:0.1mol/L醋酸缓冲液(pH=4.5)-甲醇(70:30,V/V);检测波长:270nm;流速0.5mL/min,3min即完成一次茶碱分析,与文献^[2～6](测定时间为7.3～28.4min)相比此方法具有快速,准确的特点,适用于医院茶碱血药浓度监测.

英文摘要:

      An improved and fast reversed phase high performance liquid chromatography(HPLC) method with high efficiency chromatography column, pecosphere C₁₈, 3 μm, 3.3 cm×4.6 mm, I.D(Perkin Elmer, USA) for determination of theophylline in human serum is described. The method uses a 0.1 mol/L sodium acetate buffer(pH=4.5) methanol mobile phase(70:30, V/V) with a flow rate of 0.5 mL/min and uses paracetamol as internal standard(IS). Theophylline and IS are extracted from serum using a mixture of trichloromethane: isopropanol(95:5, V/V). Quantitation is by UV absorbance at 270 nm. The intra day and inter day determination of precision for theophylline(5.27 μg/mL) varies from 2.5% to 5.4% and 2.1% to 5.0% for theophylline(10.50 μg/mL), respectively. The average recovery is more than 87.3%(n=5). Analysis time is approximately 3 min for theophylline and internal standard, it is faster than the time of reported methods(analysis times are approximately 7.3～28.4 min).