用MALDI-TOF-MS法快速测定天花粉蛋白分子量

Rapid Molecular Weight Determination of Trichosanthin by MALDITOFMS

中文关键词:  基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)  天花粉蛋白(TCS)

英文关键词:matrix-assisted laser desorption ionization time-of-flight mass spectrometry  trichosanthin

中文摘要:

      采用MALDI-TOF-MS法快速测定了天花粉蛋白的分子量,并讨论和对比了三种不同基质对其影响,认为用基质芥子酸是最佳适宜条件.实验结果表明本方法优于其它传统的测定生物大分子分子量方法.

英文摘要:

      The molecular weight of trichosanthin was determinated rapidly by MALDI-TOF-MS. The effects of three kinds of matrices were compared and diccussed, sinapinic acid was approved to be the best matrix for trichosanthia. The experiment results showed that MALDI-TOF-MS me thod was superior to the traditional method of molecular weight determination of biological macro molecules.