毛细管电泳环糊精添加剂立体选择性差异的研究

A Study of Recognition Mechanism of Enantiomeric Separation of Tulobuterol in Cyclodextrinmediated Capillary Zone Electrophoresis

中文关键词:  毛细管区带电泳  环糊精  妥布特罗  手性分离

英文关键词:capillary zone electrophoresis  cyclodextrin  tulobuterol  enantiomeric separation

中文摘要:

      对在毛细管区带电泳环糊精手性拆分体系中手性药物妥布特罗的分离机理进行了探讨.分别测定了α、β、γ-环糊精和二甲基、三甲基、羟丙基-β-环糊精与药物对映体形成包结配合物的稳定常数以及手性拆分过程中的热力学函数的变化,并对数据进行了分析.

英文摘要:

      The mechanism of enantioselectivity in cyclodextrinmediated capillary zone electrophoresis (CD-CZE) on basic drug tulobuterol was discussed. The equilibrium constants of hostguest complexion of tulobuterol enantiomers with α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, 2,6-dimethyl-β-cyclodextrin, 2,3,6-trimethyl-β-cyclodextrin and 2-hydroxy-propyl-β-cyclodextrin, and the thermodynamic parameters for chiral recognition procedure were determined respectively. Selectivity calculated from the ratio of binding constants of two enantiomers is found to be in the same order of maximum electrophortic mobility difference between the enantiomers responding with cyclodextrin additive. The experimental value of optimal concentration of cyclodextrin agrees with the one calculated from Author the equation C_opt=1/(K₁K₂)^1/2.