高效液相色谱梯度洗脱中弱溶剂的在线净化

On-Line Cleanup of Weak Solvent in Gradient Elution

中文关键词:  高效液相色谱  梯度洗脱  预柱  在线净化

英文关键词:high-performance liquid chromatography  gradient elution  precolumn  on-line cleanup

中文摘要:

      利用一个在线净化预柱减少了高压梯度洗脱中由于试剂纯度不够造成的基线噪音。预柱的位置在溶剂泵A(弱溶剂)和流动相混合器之间,由一个六通阀来控制。在线净化结果良好,有效地除去了空白梯度色谱图中的杂质峰,提高了梯度洗脱的分离重现性。该方法对开展梯度洗脱有实用价值。

英文摘要:

      Baseline noise in high-pressure gradient elution caused by impurities in weak aqueous solvent (solvent A) was improved with an on-line cleanup precolumn placed between the solvent pump A (weak solvent) and the mobile phase mixer. The procedure was implemented by using a sixport valve. With the various TEAP, H₂O/ACN, MeOH combinations examined, results obtained in the work indicate that the cleanup method is sufficiently effective and reproducible.