甲基化聚胺型硅基强阴离子交换剂用于生物大分子的分离

Separation of Biopolymers with Methylated Polyamine Encapsulated Silica based Strong Anion Exchanger

中文关键词:  甲基化聚胺型阴离子交换剂  生物大分子  高效液相色谱

英文关键词:methylated polyamine encapsulated anion exchanger  biopolymer  HPLC

中文摘要:

      用聚胺键合法合成了聚胺型碱性硅胶基质,进一步甲基化后制得了强阴离子交换剂.该强阴离子交换剂将硅基离子交换剂和树脂型离子交换剂的优点有机地结合起来,具有稳定性好,使用pH值范围宽(pH1～12),柱渗透性好的优点.可理想地用于生物大分子如蛋白质,多肽和核酸等的分离.

英文摘要:

      By reaction of oligopolyamine, epichlorohydrin alternately with λ chloropropyl triethoxyl silane bonded silica phase, and methylated afterwards, a novel silica based strong anion exchanger was prepared. This anion exchanger possesses the advantages of resin based anion exchangers and silica based anion exchangers for its high ion exchange capacity, good column permeability, being durable to much concentrated buffer and wide pH range from pH 1 to 12, and the unspecific interaction of silanol to solutes being eliminated. This strong anion exchanger can be used successfully in the separation of biopolymers. Proteins, peptides and enzymatic mouse brain RNA have being separated.