高效液相色谱法分析两种不同溴取代芴

Determination of Different Bromine-Substituted Fluorene by High Performance Liquid Chromatography

中文关键词:  2-溴芴  2,7-二溴芴  高效液相色谱法

英文关键词:2-bromofluorene  2,7-dibromofluorene  HPLC

中文摘要:

      建立了一种操作简单、适用于两种不同溴取代芴（2-溴芴和2，7-二溴芴）的高效液相色谱分析方法.采用反相C18柱，乙腈-水（体积比80∶20）为流动相，流速为1.3 mL/min，以270 nm为检测波长，外标定量测定2-溴芴和2，7-二溴芴的含量.在此条件下，2-溴芴和2，7-二溴芴在0.01~0.12 mg/mL范围内呈线性关系，相关系数均为0.999 9，平均回收率分别为106.7%和100.1%，相对标准偏差分别为0.72%和0.87%.方法操作简单，结果准确，适用于以上两种主要溴取代芴的分析检测.

英文摘要:

      A high performance liquid chromatographic(HPLC) method was set up for the monitoring of two different bromine-substituted fluorenes(2-bromofluorene and 2,7-dibromofluorene). The method consisted of a C18 column, and an acetonitrile-water(80:20,V:V)mixture was used as the mobile phase, the detection was at 270 nm with a flow rate of 1.3 mL/min. The linear range was 0.01~0.12 mg/mL, the correlation coefficients were 0.999 9, and the average recoveries were 106.7% and 100.1% respectively, with the relative standard deviations of 0.72% and 0.87%. The method is simple in operation and accurate in results, and is suitable for the analysis and detection of more than two different bromine-substituted fluorenes.