基于MnO2/石墨烯纳米材料的表面辅助激光解吸电离质谱在内毒素快速鉴定中的应用

MnO2/Graphene Nanomaterials-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Rapid Identification of Endotoxin

中文关键词:  MnO₂/石墨烯纳米复合材料  表面辅助激光解吸电离质谱  内毒素  快速鉴定

英文关键词:MnO₂/graphene nanocomposites  SALDI-MS  endotoxin  rapid identification

中文摘要:

      利用大肠埃希菌（E.coli O111：B4）中提取精制的内毒素（Control standard endotoxin，CSE）为研究对象，以MnO₂/石墨烯（MnO₂/G）纳米复合材料为基质，建立了一种基于MnO₂/G纳米材料的表面辅助激光解吸电离质谱（Surface-assisted laser desorption ionization mass spectrometry，SALDI-MS）的内毒素检测新方法.利用SALDI-MS方法可实现对不同注射液和饮用水中内毒素的快速鉴定与定量分析.与传统的鲎试剂检测方法相比，基于MnO₂/G纳米复合材料的SALDI-MS方法具有操作简便、灵敏度高、分辨率高、检测速度快、高通量和耐盐性好等优点，有望应用于更多食品和药品中细菌内毒素的高通量快速筛查.

英文摘要:

      A new method for the surface-assisted laser desorption ionization mass spectrometry (SALDI-MS) was developed using MnO₂/graphene (MnO₂/G) nanocomposites as the matrix. The control standard endotoxin (CSE) extracted from Escherichia coli (E.coli O111:B4) was used as the target and the SALDI-MS method for the analysis of CSE was set up, which could be successfully applied to the rapid identification and quantitative analysis of endotoxin in different injections and drinking water. Compared with the traditional method of tachypleus amebocyte lysate (TAL), the MnO₂/G nanocomposites-based SALDI-MS method is of shows high sensitivity, high mass resolution, rapid analysis, high throughput, and good salt tolerance. The successful applications suggest the potential of the SALDI-MS for the rapid high-throughput screening of CSE in foods and drugs.