高效液相色谱-串联质谱法测定毛发中40种常见精神药物

Determination of 40 Drugs in Human Hair by High Performance Liquid Chromotography Tandem Mass Spectroscopy

中文关键词:  毛发  40种常见精神药物  高效液相色谱-串联质谱

英文关键词:human hair  40 drugs  HPLC-MS/MS

中文摘要:

      建立了一种高效液相色谱-串联质谱法（HPLC-MS/MS）测定毛发中40种常见精神药物的方法，并应用于实际毛发样本的检测.方法以40种精神药物标准品建立的标准曲线定量，使用冷冻研磨机磨碎毛发样本，以甲醇为溶剂在70℃下超声提取2 h，使用氮吹仪浓缩提取液，加入100 μL甲醇定容后，HPLC-MS/MS上机测定.结果显示方法对于40种常见精神药物的回收率为77.3%~94.3%，在线性范围（9~770 ng/g）内相关系数大于0.942，检出限为0.01~0.8 ng/g.测定方法快速、灵敏、准确，可对毛发中40种常见精神药物进行同时定性和定量检测.

英文摘要:

      A method for screening 40 drugs in human hair by high performance liquid chromotography tandem mass spectroscopy (HPLC-MS/MS) has been established and applied for the detection of real hair samples. In this method, the linearity relationship was studied using the standard samples of 40 drugs, and human hair samples, which were ground into powder using a freeze grinder, extracted in methanol at 70℃ under ultrasonic extraction for 2 h. The concentrated extract was reconstituted to 100 μL by methanol for HPLC-MS/MS analysis. Result shows the recovery rate for 40 drugs of the method was between 77.3%~93.3%, the correlation coefficient was above 0.942 in the linear range of 9~770 ng/g, and the limit of detection was 0.01~0.8 ng/g. The method is rapid, sensitive, accurate, and can be used to the qualitative and quantitative analysis of 40 drugs in human hair.