固相支撑液液萃取-气相色谱质谱法测定血液中5种镇静安眠类药物

Analysis of Five Hypnotic Sedative Drugs in Blood by Gas Chromatography-Mass Spectrometry with Supported Liquid Extraction

中文关键词:  法医毒物分析  镇静安眠类药物  固相支撑液液萃取  气相色谱-质谱联用仪

英文关键词:forensic toxicology analysis  sedative hypnotic drugs  supported liquid extraction  GC-MS

中文摘要:

      建立了固相支撑液液萃取（SLE）-气相色谱质谱法（GC/MS）提取检验血液中阿米替林、舒乐安定、咪达唑仑、异丙嗪和氯丙嗪5种镇静安眠类药物的方法，对提取条件进行优化，浓缩后的样品溶液使用气相色谱-质谱联用仪分析.5种镇静安眠类药物的最佳提取条件：在pH 10下用5.0 mL乙酸乙酯提取，5种安眠镇静类药物在0.5~20 μg/mL范围内线性关系良好，相关系数在0.998 1~0.999 5之间，最低检出限为0.5 μg/mL，在血液中的添加回收率在70.1%~90.8%之间，变异系数均小于5.3%.方法操作简便、快捷、回收率高，可用于常见镇静安眠类药物的检验.

英文摘要:

      To establish a gas chromatography-mass spectrometry(GC/MS) method with supported liquid extraction(SLE) for the determination of common sedative hypnotic drugs in blood. Five sedative hypnotic drugs, amitriptyline, estazolamum, midazolam, promethazine and chlorpromazine, were determined by GC/MS after being processed with SLE,and experimental conditions were optimized. After adding five sedative hypnotic drugs in the blank blood,they were separated and determined by SLE-GC/MS. Optimal experimental conditions were:pH 10 and 5.0 mL of ethyl acetate as the extraction solvent. The results showed a good linearity among the concentrations of 0.5~20 μg/mL of the five drugs and the peak areas with correlation coefficients of 0.998 1~0.999 5,the limits of quantification(LOQ) were approximately 0.5 μg/mL,the average recoveries were 70.1%~90.8%,and the coefficient of variation was less than 5. 3%. The method is simple, rapid, with a high recovery, and it can be used in the determination of common sedative hypnotic drugs in blood.