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液相色谱-串联质谱法测定食品中甜味剂和抗氧化剂 |
Simultaneous Determination of Sweeteners and Antioxidants in Food by Liquid Chromatography-Tandem Mass Spectrometry |
投稿时间:2015-05-04 修订日期:2015-07-09 |
DOI: |
中文关键词: 液相色谱 四级杆飞行时间串联质谱 甜味剂 抗氧化剂 食品 |
英文关键词:liquid chromatography quadrupole time-of-flight tandem mass spectrometry sweetener antioxidant food |
基金项目:国家自然基金(21275029),福州大学科技启动专项基金(600852) |
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中文摘要: |
建立了液相色谱-四级杆飞行时间串联质谱联用技术同时测定4种甜味剂(安赛蜜、糖精钠、甜蜜素、阿斯巴甜)及2种抗氧化剂(叔丁基对苯二酚、丁基羟基茴香醚)的方法. 试验采用Extend-C18色谱柱分离与ESI(-)检测,以乙腈-1 mmol/L 乙酸铵为流动相梯度洗脱,在6 min内实现6种目标物的快速分离,检测限为0.250 0~5.00 0 ng/mL,日间精密度小于10.71%(n=3),液态样品平均回收率为83.24%~118.3%. 方法准确、灵敏,可快速检测食品中的甜味剂和抗氧化剂. |
英文摘要: |
A method for the simultaneous analysis of four sweeteners (acesulfame-K, sodium saccharin, sodium cyclamate and aspartame) and two antioxidants (tert-butylhydroquinone and butylated hydroxyanisole) by liquid chromatography-quadrupole time-of-flight mass spectrometry has been established. An extend-C18 chromatographic column and ESI (-) were used, respectively. Six compounds were separated rapidly in 6 min with 1 mmol/L of ammonium acetate-acetonitrile as the mobile phase with gradient elution. Results showed that the limit of detection ranged from 0.250 0 to 5.000 ng/mL, and the relative standard deviation of day-to-day was less than 10.71% (n=3). This assay was suitable to liquid sample detection, with average recoveries in the range of 83.24%~118.3%. The proposed method with good accuracy and high sensitivity can be applied to the rapid analysis of sweeteners and antioxidants in food. |
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