大果白刺游离脂肪酸的柱前衍生高效液相色谱-荧光检测法分析

Analysis of Fatty Acids in Nitraria Roborowskii Kom by Pre-Column Derivatization Coupled with High Performance Liquid Chromatography with Fluorescence Detection

中文关键词:  大果白刺  脂肪酸  柱前衍生  高效液相色谱-荧光检测法

英文关键词:Nitraria roborowskii Kom  fatty acid  pre-column derivatization  HPLC-FLD

中文摘要:

      采用2-（11H-苯咔唑）乙基对甲苯磺酸酯（BCETS）为柱前荧光衍生试剂，通过梯度洗脱使得18种脂肪酸在BDS-C8柱上得到良好的分离。方法应用于大果白刺不同部位中游离脂肪酸的分析，结果表明大果白刺的果皮果肉和叶子中均含有大量的不饱和脂肪酸，其总不饱和脂肪酸含量分别为70.74%和73.47%。大果白刺种子中不饱和脂肪酸的含量相对较少，仅占总脂肪酸含量的57.21%，其不饱和脂肪酸组成主要是 C18：1（油酸）和C18：2（亚油酸）。其中，大果白刺的果皮果肉中，不饱和脂肪酸主要是C18：1、C18：2和

英文摘要:

      In this study, 18 kinds of fatty acids was separated on a C8 column by gradient elute using 2-(11H-benzo[a]carbazol-11-yl) ethyl4-methylbenzenesul-fonate(BCETS) as pre-column fluorescent derivatization reagent. The developed method was applied to analyze fatty acid composition in different fractions of Nitraria roborowskii Kom. The result showed that there existed abundant of unsaturated fatty acids in the parts of sarcocarps and leaves with percentages of 70.74% and 73.47%, respectively. The unsaturated fatty acids in seeds were relatively less than those in sarcocarps and leaves, which contributed only 57.21% to the total fatty acids. The composition of unsaturated fatty acids were mainly oleic and linoleic acids. The main unsaturated fatty acids in sarcocarps were oleic, linoleic and linolenic acids. The main unsaturated fatty acid in leaves was linolenic acid, and its percentage could reach 48.34% of the total fatty acids. To the best of our knowledge, this is the first time to study the fatty acid in N. roborowskii Kom, which can provide data support for its further development and application in food, medicine and quantity control.