指示离子Cd2+测定维生素B1的方波极谱法研究

Determination of Vitamin B1 with Indicative Ion Cd2+ by Square Wave Polarography

中文关键词:  方波极谱  指示离子  维生素B1  镉离子

英文关键词:square wave polarography  indication ion  V_B1  Cd²⁺

中文摘要:

      根据Cd²⁺对维生素B1（V_B1）的指示作用，建立了用方波极谱法测定V_B1的新方法. 在氨-氯化铵缓冲溶液中，V_B1不出峰，加入Cd²⁺后在-0.856 V（vs.SCE）处出现1个固定还原峰，并在-0.360 V（vs.SCE）处可见1个峰高与V_B1浓度呈正比的还原峰. 依据该还原峰峰高的变化规律可测定V_B1的含量. V_B1在1.35 μg/mL~1.35 mg/mL的范围内有良好的线性关系，检出限可达0.13 μg/mL，r为0.999 7. 平均加样回收率为99.6%（n=9），RSD为0.9%. 利用方法测定营养胶囊中V_B1的含量，效果理想，可作为V_B1含量的测定方法.

英文摘要:

      A new method with Cd²⁺ as the indication ion, has been established for the determination of Vitamin B1 by using square polarography. No reduction wave of V_B1 appeared in the buffer solution of NH₃-NH₄Cl. When Cd²⁺ was added to the above solution, the non-electro-active V_B1 engendered a new peak at -0.360 V. The height of the peak is directly proportional to the concentration of V_B1.The linear range of V_B1 was 1.35 μg/mL~1.35 mg/mL and the determination limit was 0.13 μg/mL,r=0.9997. The mean recoveries was 99.6%(n=9), RSD=0.9%. This method is reliable,accurate and is very suitable for the determination of vitamin B1.