翻白草中鞣质的微波提取工艺研究

Microwave-Assisted Extraction of Tanning in Potentilla Discolor Bunge

中文关键词:  翻白草  鞣质  微波提取

英文关键词:potentilla discolor bunge  tanning  microwave-assisted extraction

中文摘要:

      采用微波辅助提取方法，以提取剂浓度、pH值、液固比、微波提取时间、温度等为考察因素，并以提取物中总鞣质的提取率为评价指标，使用磷钼钨酸-干酪素分光光度法为定量测定方法，在单因素实验的基础上，通过正交实验优化翻白草中鞣质的提取工艺. 实验结果表明，最佳提取条件为：使用70%甲醇作为提取剂、液固比40：1、提取液pH为8.0、提取温度为40℃、提取时间为10 min. 还对翻白草不同部位（根、茎、叶）的鞣质含量进行分析，发现根部中鞣质的含量最大，其次为茎和叶. 此外，采用高效液相色谱法对翻白草中鞣质的种类进行定性分析.

英文摘要:

      The single factor experiment and orthogonal experiment has been used to optimize the process for microwave-assisted extraction of tanning in potentilla discolor bunge, using the extractant concentration, pH, liquid-solid ratio, microwave time and extraction temperature as the investigation factors. As the assessment index, the extraction rates of tanning were detected by the phosphotungstomolybdate acid-casein spectrophotometry. The optimum conditions for the process were as follows: 70% methanol (pH=8.0) was used as the extraction agent, the liquid-solid ratio was 1:40, and extraction time was 10 min at 40℃. The contents of tannins in different parts of potentilla discolor bunge were also determined, showing that the content of tannins in the root was the highest, and followed by the stem and leaf. HPLC is also used in the qualitative analysis of type of tanning in potentilla discolor bunge.