邻菲啰啉计算光度法同时测定生物浸出样品中Fe（Ⅱ）和Fe（Ⅲ）

Simultaneous Determination of Fe(II) and Fe(III) in Bioleaching Samples by Computational Phenanthroline Photospectrometry

中文关键词:  铁价态组成分析  邻菲啰啉  计算光度法  生物浸出

英文关键词:ferrous and ferric composition analysis  phenanthroline  computational spectrophotometry  bioleaching

中文摘要:

      邻菲啰啉光度法常用于Fe（Ⅱ）测定，但受到试样中Fe（Ⅲ） 对测定的影响，因此不能直接用于生物浸出样品中Fe（Ⅱ）和Fe（Ⅲ） 的同时测定. 为此，基于Fe（Ⅱ）邻菲啰啉特征吸收曲线以及混合铁中Fe（Ⅲ） 对Fe（Ⅱ）测定的线性影响关系，建立了基于Fe（Ⅱ）和全铁同时测定Fe（Ⅱ）和Fe（Ⅲ）的计算光度法，并研究了生物浸出样品中典型金属离子（Cu²⁺、Ni²⁺、Cd²⁺、Co²⁺）以及试样溶解与储放对测定的影响.方法可准确地测定含铁次生矿物和生物浸出液中铁价态组成，应用于生物浸出矿渣、细胞表面中常量或微量的Fe（Ⅱ）和Fe（Ⅲ） 组成分析，具有简便快速的特点.

英文摘要:

      Phenanthroline (PNT) photospectrometry is often used for the determination of Fe(II), due to Fe(III) in the mixture interferes with the Fe(II) test system this method cannot simultaneously determine Fe(II) and Fe(III). So, a computational PNT photospectrometry for the simutaneous determination of Fe(II) and Fe(III) in bioleaching samples has been carried out. The linear effect of Fe(III) to the Fe(II) test system was first evaluated, and the interference of the typical metal ions (Cu²⁺, Ni²⁺, Cd²⁺, Co²⁺) in the bioleachates and dissolution of samples were investigated. Using this method, Fe(II) and Fe(III) of the typical secondary iron minerals and in the leachates can be determined accurately. It is easier and fast for the simultaneous analysis of Fe(II) and Fe(III) in the actual bioleaching residues and cell surface.