高效液相色谱-串联质谱法测定水果中残留的烯唑醇

Determination of Diniconazole Residue in Fruits by HPLC-MS/MS

中文关键词:  高效液相色谱-串联质谱  分散固相萃取  烯唑醇  水果

英文关键词:LC-MS/MS  DSPE  diniconazole  fruits

中文摘要:

      建立了水果中烯唑醇残留量的高效液相色谱-串联质谱（LC-MS/MS）测定方法。样品采用丙酮-正己烷液液振荡提取，经分散固相萃取（DSPE）净化后，采用ODS-C18柱为分离柱，以乙腈-0.1％甲酸溶液为流动相，采用液相色谱-串联质谱(ESI)多反应监测（MRM）正离子模式测定，外标法定量。烯唑醇在0.002～0.2 mg//L质量浓度范围内线性关系良好，方法定量下限（LOQ）为0.001 mg/kg。在0.005～0.1 mg/kg之间的3个添加浓度水平下，添加回收率为78.8 %~108.0 %，相对标

英文摘要:

      high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) analytical method for the determination of diniconazole residues in the fruits has been developed. Samples are liquid-liquid extracted with acetone - hexane and clean up with dispersive solid phase extraction (DSPE). The analyte is separated on an ODS-C18 column with acetonitrile-0.1% formic acid solution as the mobile phase.The target compound is detected by LC-MS/MS (ESI +) under the multiple reaction monitoring (MRM) mode using an external standard method. The linear relationship of this method was good between 0.002~0.2 mg/L concentration range. The limit of quantification (LOQ) was 0.001 mg/kg. The recoveries of diniconazole ranged from 78.8% to 108.0% at the three spiked levels of 0.005~0.1 mg/kg and the relative standard deviations(RSDs) were in range of 2.1%~6.9%.