β-环糊精增敏荧光法快速测定蔬菜中有机磷农药残留

Rapid Fluorometric Determination of Organophosphorus Pesticide Residues in Vegetables with β-CD Sensitizer

中文关键词:  β-环糊精,荧光法,甲胺磷,酶抑制法

英文关键词:β-cyclodextrin  spectrofluorogenic method,Methamidophos,enzyme inhibition

中文摘要:

      植物酯酶催化底物乙酸-1-萘酯分解为荧光物质1-萘酚，采用荧光法测定1-萘酚的荧光强度，由于β-环糊精对1-萘酚的包络作用，荧光强度显著增加，使检测灵敏度得以提高.该体系用于蔬菜中甲胺磷农药残留测定，相关系数R为0.997，线性范围为2~50 mg/L， 方法的检测限为0.5 mg/L.

英文摘要:

      Plant-esterase can catalyse the substrate 1-naphthyl acetate to be decomposed to a fluorescent product, 1-naphthol. The fluorescence intensity of 1-naphthol was measured using a spectrofluorometric method. The inclusion of β-cyclodextrin enhances the fluorescence intensity of 1-naphthol. Thus increases the sensitivity of the determination. using this method, the determination of methamidophos in vegetables has been performed with the linear relationship in the range of 2~50 mg/L,and the detection limit of 05 mg/L.