高效液相色谱法测定香连丸及组方药材中5种活性组分的含量

Determination of Five Active Components in Xianglianwan Pills and Comprised Medicinal Materials by High-performance Liquid Chromatography

中文关键词:  香连丸,含量测定,质量控制,高效液相色谱

英文关键词:Xianglianwan pills,content determination,quality control,HPLC

中文摘要:

      建立了香连丸及组方药材中药根碱、巴马汀、小檗碱、木香烃内酯和去氢木香内酯的含量测定方法.采用反相高效液相色谱，Hypersil ODS (5 μm, 4.6 mm × 250 mm) 色谱柱，流动相为乙腈:水（70∶30, V∶V）, 流速1.0 mL/min，柱温30 ℃，检测波长为210 nm测定木香烃内酯和去氢木香内酯的含量；采用高效液相胶束色谱，Kromasil ODS( 5 μm, 4.6 mm × 150 mm)色谱柱，流动相为0.2 mol/L NaH₂PO_{4水溶液∶7.00 mmol/L十二烷基硫酸钠:乙腈(35∶35∶30,V∶V∶V）, 流速1.0 mL/min，柱温30 ℃，检测波长为350 nm测定药根碱、巴马汀、小檗碱的含量.结果显示5种活性成分在适当的线性范围内均具有良好的线性关系（r大于0.9993），平均回收率在87.67%～102.37%之间，RSD小于3.13%；结果表明方法简便、灵敏、准确, 重现性好.}

英文摘要:

      Based on the determination of contents of active components in Xianglianwan pills and comprised medicinal materiinals, an available and practical method for controlling the quality of preparation and medicinal materials has been set up. The contents of costunolide and dehydrocostuslactone were assayed under chromatographic conditions as follows: Hypersil ODS column (5 μm, 4.6 mm × 250 mm), 30 ℃, mobile phase of acetonitrile and water (70∶30, V/V) at a flow rate of 1.0 mL/min, and the detective wavelength of 210 nm. Jatrorrhizine，palmatine，berberine in Xianglianwan pills and corresponding comprised medicinal materials were simultaneously determined under the following conditions: Kromasil ODS (5 μm, 4.6 mm × 150 mm), the column temperature 30 ℃, phase flow rate of 1.0 mL/min, and detective wavelength, 350 nm.All the compounds showed good linearity (r> 09993) in the range of test concentrations, average recoveries of the method were between 87.67% and 102.37%, RSD＜313%, the results showed that this method is simple, sensitive, accurate and reproducible.