金银花中绿原酸的微波预处理提取及检测

Extraction and Examination of Chlorogenic Acid from Flos Lonicerae by Microwave Pretreatment

中文关键词:  金银花,绿原酸,微波预处理,三波长法

英文关键词:flos lonicerae,chlorogenic acid,microwave pretreatment,three wavelength spectrophotometry

中文摘要:

      采用微波预处理法对金银花中绿原酸的提取进行优化，用三波长法测定了绿原酸的含量.设计正交试验,以绿原酸的含量为指标,考察乙醇用量、乙醇浓度和微波处理时间对金银花中绿原酸提取工艺的影响.三波长法检测波长为λ₁=280 nm，λ₂=325 nm，λ₃=380 nm，其回归方程为ΔA=0.0426C+0.0017，相关系数为R=0.998.结果表明在气化剂用量20 mL，乙醇浓度为70﹪，微波辐射70 s时绿原酸得率最高.与常用的提取和测定方法相比，此方法对设备要求不高，具有更高的准确性与精密度.

英文摘要:

      The method of microwave pretreatment is adopted to find the factors that affect the different extraction processes of Flos Lonicerae, and the three wavelength spectrophotometry is applied to measure the content of chlorogenic acid. The experimental results demostrate that the chlorogenic acid concentrations in 0~80 μg/mL obey linear relation with ΔA, when the absorbance was measured at wavelength λ₁=280 nm, λ₂=325 nm and λ₃=380 nm. The optimum extraction condition is the 20 mL of 70% ethanol and radiated 70 s in microwave. Comparing with the traditional method, the method requires less equipment and has reliable accuracy and good precision.