痕量新霉素的共振散射光谱分析

Resonance Scattering Spectral Analysis of Trace Neotamicin

中文关键词:  新霉素,十二烷基苯磺酸钠,缔合微粒,共振散射光谱法

英文关键词:resonance scattering spectra,neotamicin,sodium dodecyl benzene sulfate,ion association complex particles

中文摘要:

      在pH值5.9 NaAc-HAc缓冲介质中，十二烷基苯磺酸钠与新霉素相互作用形成粒径约为160±12 nm的缔合微粒, 在320、340、 420、 470 nm有4个共振散射峰.新霉素浓度在0.076~6.840 μｇ/mL范围内与470 nm 处的共振散射强度成正比，检出限为0.03 μｇ/mL.该法具有选择性较好、灵敏度较高、快速、简便等特点，用于市售滴耳液和尿液分析，结果满意.

英文摘要:

      In pH 5.9 NaAc-HAc buffer solution, neotamicin (NEO) interacts with sodium dodecyl benzene sulfate (SDB) to form ion association complex particles in size of about 160±12 nm. There are four RS peaks at 320, 340, 420 and 470 nm. The NEO concentration in the range of 0.076~6.840 μｇ/mL is proportional to the resonance scattering intensity at 470 nm. The detection limit is 0.03 μｇ/mL. The method has good selectivity and sensitivity, which can be applied to the determination of NEO in ear drips and urine samples, with satisfactory results.