PCR生物微流体芯片中的表面钝化技术

Surface Inactivation Technology in PCR Bio-microfluidical Chip

中文关键词:  聚合酶链式反应  微流体芯片  表面钝化

英文关键词:polymerase chain reaction(PCR)  microfluidical chip  surface inactivation

中文摘要:

      聚合酶链式反应(polymerase chain reaction,PCR)是一种重要的体外DNA扩增技术,在生物化学、分析化学以及分子生物学等领域中得到广泛的应用.基于微电子机械系统(Microelectromechanical system,MEMS)技术构建而成的PCR生物微流体芯片由于具有反应速度快、样品消耗少以及所占空间体积少等优点而倍受人们亲睐.然而,伴随之较大比表面积以及其所用的裸露衬底材料常常抑制PCR反应,从而导致PCR不能顺利进行.本文结合本试验室的研究工作,综述了PCR生物微流体芯片中为了获得"友善"的PCR扩增体系而采取的表面钝化技术,主要包括表面钝化的必要性、静力学/动力学表面钝化技术以及硅相关材料对PCR抑制的机制等等.

英文摘要:

      Polymerase chain reaction(PCR) is an important technique of DNA amplification in vitro,which has been widely applied to the field of biological chemistry,analytical chemistry and molecular biology.Great interests have been attracted onto the PCR bio-microfluidical chips fabricated by microelectromechanical system(MEMS)technology due to their many advantages such as fast reaction rate,little sample consumption and small footprint.However,its corresponding high surface-to-volume ratio and the utilized bare substrate material usually inhibit PCR amplification reaction which will lead to non-access to successful PCR amplification.In this review,the systematic description involves the surface inactivation technology used in the PCR microfluidical chip in order to acquire the "friend" surface to PCR reaction,including the necessity of surface inactivation,static/dynamic inactivation,mechanism of PCR inhibition by silicon-related materials and so on.