市售大黄及三黄片中蒽醌类活性成分的胶束电动毛细管色谱含量测定

Separation and Determination of Anthraquinones in Rhubarb and its Preparation by Micellar Electrokinetic Capillary Chromatography

中文关键词:  蒽醌  大黄  毛细管胶束电动色谱

英文关键词:Anthraquinones  Rhubarb  Micellar electrokinetic capillary chromatography

中文摘要:

      建立了胶束电动毛细管色谱分离和测定大黄及其制剂三黄片中蒽醌类活性组分的方法.考察了背景电解质pH、表面活性剂浓度、有机改性剂种类和浓度对分离的影响.实验结果表明:在缓冲液pH为9.5、SDS浓度为25mmol/L、乙氰浓度为20%时的优化条件下,大黄及三黄片中蒽醌类活性组分得到基线分离且方法具有较好的重现性.

英文摘要:

      A micellar electrokinetic capillary chromatography method was developed for the determination of anthraquinones in rhubarb and its preparation. The effects of pH value of buffer system, the concentration of sodium dodecyl sulfate (SDS), the organic modifier and its concentration on the separation selectivity were discussed. The results indicated that the anthraquinones were baseline separation under the 10 mmol/L borate buffer at pH 9.5 with 25 mmol/L SDS and 20%(V/V) acetonitrile. The calibration curves exhibited good linearity between the concentrations and the peak area of the three components. The relative standard deviations (RSD) of migration time and peak areas were 3.45% and 4.86%, respectively.