胶体金在DNA分子识别中的应用——胶体金对罗丹明B与单双链DNA识别作用的影响

The Application of Colloidal Gold in DNA Recognition

中文关键词:  胶体金  罗丹明B  DNA  分子识别

英文关键词:colloidal gold  Rhodamine B  DNA  molecular recognition

中文摘要:

      通过研究罗丹明B与单双链DNA作用过程中,胶体金加入前后的紫外可见吸收光谱的变化,发现在胶体金加入的前后,罗丹明B均以沟槽方式与单双链DNA相作用;胶体金的加入可以较大程度地增大化合物与DNA之间的相互作用.

英文摘要:

      The recognition mechanism of Rhodamine B (RB) with single-stranded DNA and double-stranded DNA in the absence and presence of colloidal gold has been investigated by using the titration of UV-Vis spectroscopy. The results indicate that a groove binding may occur between RB and DNA in the absence and/or presence of colloidal gold. Besides, it is also observed that the presence of colloidal gold can significantly facilitate the interaction between DNA and the ligands.