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高效液相色谱法测定山生柳中酚苷类成分的含量 |
Determination of Phenolic Glycosides from Salix Oritrepha by High-performance Liquid Chromatography |
投稿时间:2002-09-14 修订日期:2002-11-25 |
DOI: |
中文关键词: 山生柳 酚苷 高效液相色谱 |
英文关键词:salix oritrepha phenolic glycoside high-performance liquid chromatography |
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中文摘要: |
用高效液相色谱法测定山生柳中水杨苷、水杨酰水杨苷和木犀草素-7-O-葡萄糖苷的含量.流动相为1.5%醋酸溶液-甲醇,SymmetryC18柱,柱温30℃,流速1.1mL/min,检测波长270nm.线性浓度范围分别为527.0~105.4μg/mL,140.0~28.0μg/mL,81.0~16.2μg/mL,平均回收率是104.9%、104.6%和103.6%,RSD分别为2.0%、0.3%和0.3%. |
英文摘要: |
Salicin, Salicyloylsalicin, and Leteolin 7-O-glucoside were determined from S. Oritrepha. Methods: High-performance liquid chromatography(HPLC) on a Symmetry C18 column (4.6 mm×250 mm, 5 μm) with acetic acid-methanol gradient elution, at column temperature of 30℃, flow-rate of 1.1 mL/min and wavelength at 270 nm. Results: The contents of three compounds can be determined simultaneously from S. Oritrepha.The linear concentration ranges of these compounds were 527.0~105.4 μg/mL, 140.0~28.0 μg/mL, 81.0~16.2 μg/mL respectively. The average recoveries were 104.9%, 104.6% and 103.6% respectively, and relative standard deviations were 2.0%, 0.3% and 0.3% respectively. Conclusion: The proposed method was a simple and rapid method for simultaneous determination of the three mentioned compounds in various tissues from S. Oriterpha. |
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