串联质谱法检测血清中的左羟丙哌嗪

Determination of Levodropropizine in Serum by Tandem Gas Chromatography-Mass Spectrometry

中文关键词:  串联质谱法  左羟丙哌嗪  血清

英文关键词:tandem gas chromat ography-mass spectrometry  levodropropizine  Serum

中文摘要:

      血清样品调节成碱性后,加到硅藻土小柱上,用二氯甲烷洗脱左旋羟丙哌嗪,在微弱氮气流下吹干洗脱液,残渣以BSTFA衍生后用GC/MS进行定性定量分析.以m/z175为母离子,子离子质量扫描范围为m/z50～200,外标法定量.方法的线性范围为:0.005～4.0μg/mL,相关系数为0.99,检测低限为0.005mg/L,回收率范围为80%～102.5%,相对标准偏差范围为1.67%～4.00%.

英文摘要:

      Serum sample was made alkaline by sodium hydroxide,and then was added to minicolumn.Levo-dropropizine was eluted with methylene dichloride.The eluted organic phase was dried under nitrogen.The residue was derived by BSTFA before analyzed by Tandem GC-MS.The parention was m/z 175,and daughter ion was from m/z 50 to m/z 200.The external standard method was used for quantitative.The liner range,the correlation coefficient,the detection limit,the recoveries and RSD was 0.005~4.0 μg/mL,0.99,0.005 mg/L,80%~102.5%,1.67%~4.00%,respectively.