高效液相色谱－核磁共振联用技术及其应用

Hyphenation of High-performance Liquid Chromatography with Nuclear Magnetic Resonance and its Applications

中文关键词:  高效液相色谱  核磁共振  灵敏度  溶剂峰抑制

英文关键词:High-performance liquid chromatography  nuclear magnetic resonance sensitivity  solvent peak suppression

中文摘要:

      高效液相色谱-核磁共振(HPLC-NMR)在线联用技术是同时进行未知混合物的分离和结构鉴定的最好手段之一.详细介绍了在HPLC-NMR联用技术中1H谱的分辨率、检测限和多重溶剂峰抑制的最新进展,并简要评述了其它分离方法与NMR联用情况.

英文摘要:

      The direct on-line coupling between high performance liquid chromatography (HPLC) and proton nuclear magnetic resonance (NMR) spectroscopy is one of the most powerful techniques for simultaneous separation and structural elucidation of unknown compounds in mixtures.The resolution,detection limit and multiple solvent suppression of the 1H NMR spectra obtained in HPLC-NMR are described. Recent advances in the hyphenation between other separation methods and NMR spectroscopy are discussed.